› 1mjw

X-ray diffraction
1.95Å resolution

STRUCTURE OF INORGANIC PYROPHOSPHATASE MUTANT D42N

Released:
DOI: 10.2210/pdb1mjw/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 1mjw coloured by chain, front view.
PDB entry 1mjw coloured by chain, side view.
PDB entry 1mjw coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Three-dimensional structures of mutant forms of E. coli inorganic pyrophosphatase with Asp-->Asn single substitution in positions 42, 65, 70, and 97.
Avaeva SM, Rodina EV, Vorobyeva NN, Kurilova SA, Nazarova TI, Sklyankina VA, Oganessyan VY, Samygina VR, Harutyunyan EH
Biochemistry (Mosc) 63 671-84 (1998)
PMID: 9668207

Function and Biology Details

Reaction catalysed: 
Diphosphate + H(2)O = 2 phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141571 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inorganic pyrophosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 175 amino acids
Theoretical weight: 19.6 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7A9 (Residues: 2-176; Coverage: 99%)
Gene names: JW4185, b4226, ppa
Sequence domains: Inorganic pyrophosphatase
Structure domains: Inorganic pyrophosphatase

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
Spacegroup: R32
Unit cell:
a: 110.3Å b: 110.3Å c: 154.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.191 0.266
Expression system: Escherichia coli

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Citations

1 citations in other articles

Activation of Helicobacter pylori inorganic pyrophosphatase and the importance of Cys16 in thermostability, enzyme activation and quaternary structure.
Lee et al. (2007)

3 mentions without citation

Improving structure-based function prediction using molecular dynamics.
Glazer et al. (2009)
2 more