› 1mki

X-ray diffraction
2Å resolution

Crystal Structure of Bacillus Subtilis Probable Glutaminase, APC1040

Released:
DOI: 10.2210/pdb1mki/pdb
PDB entry 1mki coloured by chain, front view.
PDB entry 1mki coloured by chain, side view.
PDB entry 1mki coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Functional and structural characterization of four glutaminases from Escherichia coli and Bacillus subtilis.
Brown G, Singer A, Proudfoot M, Skarina T, Kim Y, Chang C, Dementieva I, Kuznetsova E, Gonzalez CF, Joachimiak A, Savchenko A, Yakunin AF
Biochemistry 47 5724-35 (2008)
PMID: 18459799

Function and Biology Details

Reaction catalysed: 
L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-128289 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaminase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 330 amino acids
Theoretical weight: 37.14 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O31465 (Residues: 1-327; Coverage: 100%)
Gene names: BSU02430, glsA, glsA1, ybgJ
Sequence domains: Glutaminase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand EDO 4 x EDO
Ligand FMT 1 x FMT
2 modified residues:
Ligand MSE 24 x MSE
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 71.332Å b: 181.482Å c: 51.488Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.212 0.245
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

Control of immune response by amino acid metabolism.
Grohmann et al. (2010)
6 more

9 mentions without citation

Conformational changes in protein loops and helices induced by post-translational phosphorylation.
Groban et al. (2006)
8 more