› 1mkt

X-ray diffraction
1.72Å resolution

CARBOXYLIC ESTER HYDROLASE, 1.72 ANGSTROM TRIGONAL FORM OF THE BOVINE RECOMBINANT PLA2 ENZYME

Released:
DOI: 10.2210/pdb1mkt/pdb
PDB entry 1mkt coloured by chain, front view.
PDB entry 1mkt coloured by chain, side view.
PDB entry 1mkt coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
1.72 A resolution refinement of the trigonal form of bovine pancreatic phospholipase A2.
Sekar K, Sekharudu C, Tsai MD, Sundaralingam M
Acta Crystallogr D Biol Crystallogr 54 342-6 (1998)
PMID: 9761901

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132751 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase A2 Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 13.81 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00593 (Residues: 23-145; Coverage: 95%)
Gene name: PLA2G1B
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU R-AXIS II
Spacegroup: P3121
Unit cell:
a: 46.78Å b: 46.78Å c: 102.89Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.195 0.284
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Crystal structures of the free and anisic acid bound triple mutant of phospholipase A2.
Sekar et al. (2003)
2 more

3 mentions without citation

3dSS: 3D structural superposition.
Sumathi et al. (2006)
2 more