› 1mky

X-ray diffraction
1.9Å resolution

Structural Analysis of the Domain Interactions in Der, a Switch Protein Containing Two GTPase Domains

Released:
DOI: 10.2210/pdb1mky/pdb
PDB entry 1mky coloured by chain, front view.
PDB entry 1mky coloured by chain, side view.
PDB entry 1mky coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Domain arrangement of Der, a switch protein containing two GTPase domains.
Robinson VL, Hwang J, Fox E, Inouye M, Stock AM
Structure 10 1649-58 (2002)
PMID: 12467572

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase Der Chain: A
Molecule details ›
Chain: A
Length: 439 amino acids
Theoretical weight: 50.54 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X1F8 (Residues: 2-439; Coverage: 100%)
Gene names: TM_1446, der, engA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PO4 5 x PO4
Ligand GDP 1 x GDP
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 62.137Å b: 77.994Å c: 96.597Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.204 0.224
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Structure-function relationships of the G domain, a canonical switch motif.
Wittinghofer et al. (2011)
10 more

13 mentions without citation

The application of FAST-NMR for the identification of novel drug discovery targets.
Powers et al. (2008)
12 more