› 1mkz

X-ray diffraction
1.6Å resolution

Crystal structure of MoaB protein at 1.6 A resolution.

Released:
DOI: 10.2210/pdb1mkz/pdb
PDB entry 1mkz coloured by chain, front view.
PDB entry 1mkz coloured by chain, side view.
PDB entry 1mkz coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The crystal structure of Escherichia coli MoaB suggests a probable role in molybdenum cofactor synthesis.
Sanishvili R, Beasley S, Skarina T, Glesne D, Joachimiak A, Edwards A, Savchenko A
J Biol Chem 279 42139-46 (2004)
PMID: 15269205

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142682 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Molybdenum cofactor biosynthesis protein B Chains: A, B
Molecule details ›
Chains: A, B
Length: 172 amino acids
Theoretical weight: 18.94 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEZ9 (Residues: 1-170; Coverage: 100%)
Gene names: JW0765, b0782, chlA2, moaB
Sequence domains: Probable molybdopterin binding domain
Structure domains: MoaB/Mog-like domain

Ligands and Environments

2 bound ligands:
Ligand SO4 7 x SO4
Ligand ACY 3 x ACY
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P321
Unit cell:
a: 69.169Å b: 69.169Å c: 126.184Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.183 0.219
Expression system: Escherichia coli BL21(DE3)

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Citations

8 citation in other articles

A widespread riboswitch candidate that controls bacterial genes involved in molybdenum cofactor and tungsten cofactor metabolism.
Regulski et al. (2008)
7 more

3 mentions without citation

Towards fully automated structure-based function prediction in structural genomics: a case study.
Watson et al. (2007)
2 more