› 1mnv

X-ray diffraction
2.6Å resolution

Actinomycin D binding to ATGCTGCAT

Released:
DOI: 10.2210/pdb1mnv/pdb
PDB entry 1mnv coloured by chain, front view.
PDB entry 1mnv coloured by chain, side view.
PDB entry 1mnv coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of actinomycin D bound to the CTG triplet repeat sequences linked to neurological diseases.
Hou MH, Robinson H, Gao YG, Wang AH
Nucleic Acids Res 30 4910-7 (2002)
PMID: 12433994

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-114195 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
ACTINOMYCIN D Chains: C, D
Molecule details ›
Chains: C, D
Length: 11 amino acids
Theoretical weight: 1.26 KDa
Source organism: Streptomyces antibioticus
Expression system: Not provided
5'-D(*AP*TP*GP*CP*TP*GP*CP*AP*T)-3' Chains: A, B
Molecule details ›
Chains: A, B
Length: 9 nucleotides
Theoretical weight: 2.73 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
3 modified residues:
Ligand DVA 4 x DVA
Ligand SAR 4 x SAR
Ligand MVA 4 x MVA

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3112
Unit cell:
a: 47.24Å b: 47.24Å c: 69.45Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.233 0.233 0.295
Expression system: Not provided

Quick links

Citations

6 review citations

Conformational changes in DNA upon ligand binding monitored by circular dichroism.
Chang et al. (2012)
5 more

8 mentions without citation

Isolation, characterization, anti-MRSA evaluation, and in-silico multi-target anti-microbial validations of actinomycin X2 and actinomycin D produced by novel Streptomyces smyrnaeus UKAQ_23.
Qureshi et al. (2021)
7 more