› 1moe

X-ray diffraction
2.6Å resolution

The three-dimensional structure of an engineered scFv T84.66 dimer or diabody in VL to VH linkage.

Released:
DOI: 10.2210/pdb1moe/pdb
PDB entry 1moe coloured by chain, front view.
PDB entry 1moe coloured by chain, side view.
PDB entry 1moe coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The crystal structure of an anti-CEA scFv diabody assembled from T84.66 scFvs in V(L)-to-V(H) orientation: implications for diabody flexibility.
Carmichael JA, Power BE, Garrett TP, Yazaki PJ, Shively JE, Raubischek AA, Wu AM, Hudson PJ
J Mol Biol 326 341-51 (2003)
PMID: 12559905

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-233673 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ig-like domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 240 amino acids
Theoretical weight: 25.82 KDa
Source organism: Mus musculus
Expression system: Mus musculus
UniProt:
  • Canonical: Q5R3X1 (Residues: 1-92, 93-98, 99-113; Coverage: 99%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU, null
Spacegroup: P41212
Unit cell:
a: 64.314Å b: 64.314Å c: 247.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.213 0.3
Expression system: Mus musculus

Quick links

Citations

4 review citations

Engineered antibody fragments and the rise of single domains.
Holliger et al. (2005)
3 more

4 mentions without citation

Development of PF-06671008, a Highly Potent Anti-P-cadherin/Anti-CD3 Bispecific DART Molecule with Extended Half-Life for the Treatment of Cancer.
Root et al. (2016)
3 more