› 1moq

X-ray diffraction
1.57Å resolution

ISOMERASE DOMAIN OF GLUCOSAMINE 6-PHOSPHATE SYNTHASE COMPLEXED WITH GLUCOSAMINE 6-PHOSPHATE

Released:
DOI: 10.2210/pdb1moq/pdb
PDB entry 1moq coloured by chain, front view.
PDB entry 1moq coloured by chain, side view.
PDB entry 1moq coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Involvement of the C terminus in intramolecular nitrogen channeling in glucosamine 6-phosphate synthase: evidence from a 1.6 A crystal structure of the isomerase domain.
Teplyakov A, Obmolova G, Badet-Denisot MA, Badet B, Polikarpov I
Structure 6 1047-55 (1998)
PMID: 9739095

Function and Biology Details

Reaction catalysed: 
L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147884 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] Chain: A
Molecule details ›
Chain: A
Length: 368 amino acids
Theoretical weight: 40.36 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P17169 (Residues: 242-609; Coverage: 60%)
Gene names: JW3707, b3729, glmS
Sequence domains: SIS domain
Structure domains: Glucose-6-phosphate isomerase like protein; domain 1

Ligands and Environments

5 bound ligands:
Ligand GLP 1 x GLP
Ligand SO4 5 x SO4
Ligand NA 1 x NA
Ligand MES 1 x MES
Ligand MRD 1 x MRD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: R32
Unit cell:
a: 143.7Å b: 143.7Å c: 173.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.185 not available
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Cytoplasmic steps of peptidoglycan biosynthesis.
Barreteau et al. (2008)
6 more

16 mentions without citation

A graph-theory algorithm for rapid protein side-chain prediction.
Canutescu et al. (2003)
15 more