› 1mp5

X-ray diffraction
2.75Å resolution

Y177F VARIANT OF S. ENTERICA RmlA

Released:
DOI: 10.2210/pdb1mp5/pdb
PDB entry 1mp5 coloured by chain, front view.
PDB entry 1mp5 coloured by chain, side view.
PDB entry 1mp5 coloured by chain, top view.
Source organism: Salmonella enterica
Primary publication:
Expanding pyrimidine diphosphosugar libraries via structure-based nucleotidylyltransferase engineering.
Barton WA, Biggins JB, Jiang J, Thorson JS, Nikolov DB
Proc Natl Acad Sci U S A 99 13397-402 (2002)
PMID: 12374866

Function and Biology Details

Reaction catalysed: 
dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-alpha-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150737 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-1-phosphate thymidylyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 292 amino acids
Theoretical weight: 32.41 KDa
Source organism: Salmonella enterica
Expression system: Escherichia coli
UniProt:
  • Canonical: P26393 (Residues: 1-292; Coverage: 100%)
Gene names: STM2095, rfbA, rmlA
Sequence domains: Nucleotidyl transferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

1 bound ligand:
Ligand UPG 4 x UPG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 91.7Å b: 111.22Å c: 131.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.29
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Natural-product sugar biosynthesis and enzymatic glycodiversification.
Thibodeaux et al. (2008)
7 more

2 mentions without citation

The structural biology of enzymes involved in natural product glycosylation.
Singh et al. (2012)
1 more