› 1mpe

Solution NMR

Ensemble of 20 structures of the tetrameric mutant of the B1 domain of streptococcal protein G

Released:
DOI: 10.2210/pdb1mpe/pdb
PDB entry 1mpe coloured by chain, ensemble of 21 models, front view.
PDB entry 1mpe coloured by chain, ensemble of 21 models, side view.
PDB entry 1mpe coloured by chain, ensemble of 21 models, top view.
Source organism: Streptococcus sp. 'group G'
Primary publication:
Core mutations switch monomeric protein GB1 into an intertwined tetramer.
Kirsten Frank M, Dyda F, Dobrodumov A, Gronenborn AM
Nat Struct Biol 9 877-85 (2002)
PMID: 12379842

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139125 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin G-binding protein G Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 56 amino acids
Theoretical weight: 6.3 KDa
Source organism: Streptococcus sp. 'group G'
Expression system: Escherichia coli
UniProt:
  • Canonical: P06654 (Residues: 228-282; Coverage: 13%)
Gene name: spg
Sequence domains: B domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

9 review citations

The unfolding story of three-dimensional domain swapping.
Rousseau et al. (2003)
8 more

2 mentions without citation

Protein acrobatics in pairs--dimerization via domain swapping.
Gronenborn AM. (2009)
1 more