› 1msi

X-ray diffraction
1.25Å resolution

STRUCTURE OF ANTIFREEZE GLYCOPROTEIN QAE(HPLC 12)

Released:
DOI: 10.2210/pdb1msi/pdb
PDB entry 1msi coloured by chain, front view.
PDB entry 1msi coloured by chain, side view.
PDB entry 1msi coloured by chain, top view.
Source organism: Zoarces americanus
Primary publication:
Structural basis for the binding of a globular antifreeze protein to ice.
Jia Z, DeLuca CI, Chao H, Davies PL
Nature 384 285-8 (1996)
PMID: 8918883

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148668 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type-3 ice-structuring protein HPLC 12 Chain: A
Molecule details ›
Chain: A
Length: 70 amino acids
Theoretical weight: 7.42 KDa
Source organism: Zoarces americanus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19614 (Residues: 2-64; Coverage: 96%)
Structure domains: Antifreeze-like/N-acetylneuraminic acid synthase C-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 32.328Å b: 38.893Å c: 45.483Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.225
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Psychrophilic enzymes: hot topics in cold adaptation.
Feller et al. (2003)
21 more

24 mentions without citation

A graph-theory algorithm for rapid protein side-chain prediction.
Canutescu et al. (2003)
23 more