› 1mtl

X-ray diffraction
2.8Å resolution

Non-productive MUG-DNA complex

Released:
DOI: 10.2210/pdb1mtl/pdb
PDB entry 1mtl coloured by chain, front view.
PDB entry 1mtl coloured by chain, side view.
PDB entry 1mtl coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of a DNA base-excision product resembling a cisplatin inter-strand adduct.
Barrett TE, Savva R, Barlow T, Brown T, Jiricny J, Pearl LH
Nat Struct Biol 5 697-701 (1998)
PMID: 9699633

Function and Biology Details

Reaction catalysed: 
Specifically hydrolyzes mismatched double-stranded DNA and polynucleotides, releasing free uracil
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114204 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
G/U mismatch-specific DNA glycosylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 168 amino acids
Theoretical weight: 18.7 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A9H1 (Residues: 1-168; Coverage: 100%)
Gene names: JW3040, b3068, mug, ygjF
Sequence domains: Uracil DNA glycosylase superfamily
Structure domains: Uracil-DNA glycosylase-like domain
5'-D(*CP*GP*CP*GP*AP*GP*(AAB)P*TP*CP*GP*CP*G)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 12 nucleotides
Theoretical weight: 3.57 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: P212121
Unit cell:
a: 67.62Å b: 84.86Å c: 97.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.28 0.206 0.286
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

Unusual DNA duplex and hairpin motifs.
Chou et al. (2003)
6 other articles

1 mention without citation

Recent advances in the structural mechanisms of DNA glycosylases.
Brooks et al. (2013)