› 1mto

X-ray diffraction
3.2Å resolution

Crystal structure of a Phosphofructokinase mutant from Bacillus stearothermophilus bound with fructose-6-phosphate

Released:
DOI: 10.2210/pdb1mto/pdb
PDB entry 1mto coloured by chain, front view.
PDB entry 1mto coloured by chain, side view.
PDB entry 1mto coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Reversible ligand-induced dissociation of a tryptophan-shift mutant of phosphofructokinase from Bacillus stearothermophilus.
Riley-Lovingshimer MR, Ronning DR, Sacchettini JC, Reinhart GD
Biochemistry 41 12967-74 (2002)
PMID: 12390023

Function and Biology Details

Reaction catalysed: 
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132577 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent 6-phosphofructokinase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 319 amino acids
Theoretical weight: 34.17 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00512 (Residues: 1-319; Coverage: 100%)
Gene names: pfk, pfkA
Sequence domains: Phosphofructokinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand F6P 8 x F6P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 110.682Å b: 106.872Å c: 119.588Å
α: 90° β: 113.98° γ: 90°
R-values:
R R work R free
0.189 0.18 0.28
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Dissection of the network of interactions that links RNA processing with glycolysis in the Bacillus subtilis degradosome.
Newman et al. (2012)
5 more

4 mentions without citation

PocketAnnotate: towards site-based function annotation.
Anand et al. (2012)
3 more