› 1mtp

X-ray diffraction
1.5Å resolution

The X-ray crystal structure of a serpin from a thermophilic prokaryote

Released:
DOI: 10.2210/pdb1mtp/pdb
PDB entry 1mtp coloured by chain, front view.
PDB entry 1mtp coloured by chain, side view.
PDB entry 1mtp coloured by chain, top view.
Source organism: Thermobifida fusca
Primary publication:
The 1.5 A crystal structure of a prokaryote serpin: controlling conformational change in a heated environment.
Irving JA, Cabrita LD, Rossjohn J, Pike RN, Bottomley SP, Whisstock JC
Structure 11 387-97 (2003)
PMID: 12679017

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175292 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serpin domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 34.98 KDa
Source organism: Thermobifida fusca
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47NK3 (Residues: 1-323; Coverage: 88%)
Gene name: Tfu_1933
Sequence domains: Serpin (serine protease inhibitor)
Structure domains:
Serpin domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 43 amino acids
Theoretical weight: 4.79 KDa
Source organism: Thermobifida fusca
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47NK3 (Residues: 324-366; Coverage: 12%)
Gene name: Tfu_1933

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 41.936Å b: 65.595Å c: 124.737Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.223
Expression system: Escherichia coli

Quick links

Citations

6 review citations

An overview of the serpin superfamily.
Law et al. (2006)
5 more

3 mentions without citation

Exploring key orientations at protein-protein interfaces with small molecule probes.
Ko et al. (2013)
2 more