› 1mu4

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE AT 1.8 ANGSTROMS OF THE BACILLUS SUBTILIS CATABOLITE REPRESSION HISTIDINE CONTAINING PROTEIN (CRH)

Released:
DOI: 10.2210/pdb1mu4/pdb
PDB entry 1mu4 coloured by chain, front view.
PDB entry 1mu4 coloured by chain, side view.
PDB entry 1mu4 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Dimerization of Crh by reversible 3D domain swapping induces structural adjustments to its monomeric homologue Hpr.
Juy M, Penin F, Favier A, Galinier A, Montserret R, Haser R, Deutscher J, Böckmann A
J Mol Biol 332 767-76 (2003)
PMID: 12972249

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-126550 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HPr-like protein Crh Chains: A, B
Molecule details ›
Chains: A, B
Length: 87 amino acids
Theoretical weight: 9.58 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O06976 (Residues: 1-85; Coverage: 100%)
Gene names: BSU34740, crh, yvcM
Sequence domains: PTS HPr component phosphorylation site
Structure domains: HPr-like

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF
Spacegroup: P4322
Unit cell:
a: 68.85Å b: 68.85Å c: 119.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.179 0.197
Expression system: Escherichia coli

Quick links

Citations

2 review citations

How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.
Deutscher et al. (2006)
1 more

7 mentions without citation

Proton assisted recoupling and protein structure determination.
De Paëpe et al. (2008)
6 more