› 1mug

X-ray diffraction
1.8Å resolution

G:T/U MISMATCH-SPECIFIC DNA GLYCOSYLASE FROM E.COLI

Released:
DOI: 10.2210/pdb1mug/pdb
PDB entry 1mug coloured by chain, front view.
PDB entry 1mug coloured by chain, side view.
PDB entry 1mug coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of a G:T/U mismatch-specific DNA glycosylase: mismatch recognition by complementary-strand interactions.
Barrett TE, Savva R, Panayotou G, Barlow T, Brown T, Jiricny J, Pearl LH
Cell 92 117-29 (1998)
PMID: 9489705

Function and Biology Details

Reaction catalysed: 
Specifically hydrolyzes mismatched double-stranded DNA and polynucleotides, releasing free uracil
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141878 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
G/U mismatch-specific DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 168 amino acids
Theoretical weight: 18.7 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A9H1 (Residues: 1-168; Coverage: 100%)
Gene names: JW3040, b3068, mug, ygjF
Sequence domains: Uracil DNA glycosylase superfamily
Structure domains: Uracil-DNA glycosylase-like domain

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P212121
Unit cell:
a: 40.01Å b: 49.05Å c: 91.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

29 review citations

Uracil in DNA--occurrence, consequences and repair.
Krokan et al. (2002)
28 more

14 mentions without citation

Recent advances in the structural mechanisms of DNA glycosylases.
Brooks et al. (2013)
13 more