1muh

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF TN5 TRANSPOSASE COMPLEXED WITH TRANSPOSON END DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-114205 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transposase for transposon Tn5 Chain: A
Molecule details ›
Chain: A
Length: 481 amino acids
Theoretical weight: 53.85 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46731 (Residues: 1-476; Coverage: 100%)
Gene names: tnp, tnpA
Sequence domains:
Structure domains:
DNA TRANSFERRED STRAND Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.21 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA NON-TRANSFERRED STRAND Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.05 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6522
Unit cell:
a: 113.7Å b: 113.7Å c: 228.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.204 0.267
Expression systems:
  • Escherichia coli
  • Not provided