› 1mul

X-ray diffraction
2.3Å resolution

Crystal structure of the E. coli HU alpha2 protein

Released:
DOI: 10.2210/pdb1mul/pdb
PDB entry 1mul coloured by chain, front view.
PDB entry 1mul coloured by chain, side view.
PDB entry 1mul coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Evidence of a thermal unfolding dimeric intermediate for the Escherichia coli histone-like HU proteins: thermodynamics and structure.
Ramstein J, Hervouet N, Coste F, Zelwer C, Oberto J, Castaing B
J Mol Biol 331 101-21 (2003)
PMID: 12875839

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-142269 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-binding protein HU-alpha Chain: A
Molecule details ›
Chain: A
Length: 90 amino acids
Theoretical weight: 9.55 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0ACF0 (Residues: 1-90; Coverage: 100%)
Gene names: JW3964, b4000, hupA
Sequence domains: Bacterial DNA-binding protein
Structure domains: IHF-like DNA-binding proteins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I222
Unit cell:
a: 31.09Å b: 55.34Å c: 117.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.256 0.234 0.267
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

IHF and HU: flexible architects of bent DNA.
Swinger et al. (2004)
4 more

13 mentions without citation

Conservation of the three-dimensional structure in non-homologous or unrelated proteins.
Sousounis et al. (2012)
12 more