› 1mvu

X-ray diffraction
1.78Å resolution

SINGLE CHAIN FV OF C219 HEAVY CHAIN V101L MUTANT IN COMPLEX WITH SYNTHETIC EPITOPE PEPTIDE

Released:
DOI: 10.2210/pdb1mvu/pdb
PDB entry 1mvu coloured by chain, front view.
PDB entry 1mvu coloured by chain, side view.
PDB entry 1mvu coloured by chain, top view.
Source organisms:
Primary publication:
Single Chain C219 V(101H)L Mutant Antibody Complexed with a Helical Peptide
Chan DCM, Hirama T, Mackenzie CR, Kuntz DA, Rose DR
To be published

Function and Biology Details

Reactions catalysed: 
ATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2)
ATP + H(2)O + xenobiotic(Side 1) = ADP + phosphate + xenobiotic(Side 2)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-149251 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 114 amino acids
Theoretical weight: 12.53 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6KB05 (Residues: 137-249; Coverage: 44%)
Gene name: scFv B8E5
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 121 amino acids
Theoretical weight: 13.44 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q505N9 (Residues: 20-124, 125-138; Coverage: 27%)
Gene name: Igh
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
ATP-dependent translocase ABCB1 Chain: P
Molecule details ›
Chain: P
Length: 13 amino acids
Theoretical weight: 1.47 KDa
Source organism: Cricetulus griseus
Expression system: Not provided
UniProt:
  • Canonical: P21448 (Residues: 1210-1222; Coverage: 1%)
Gene names: ABCB1, PGP1, PGY1

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 41.733Å b: 50.967Å c: 58.636Å
α: 90° β: 93.89° γ: 90°
R-values:
R R work R free
0.2 0.197 0.223
Expression systems:
  • Escherichia coli
  • Not provided

Quick links