› 1mw3

X-ray diffraction
2Å resolution

Amylosucrase soaked with 1M sucrose

Released:
DOI: 10.2210/pdb1mw3/pdb
PDB entry 1mw3 coloured by chain, front view.
PDB entry 1mw3 coloured by chain, side view.
PDB entry 1mw3 coloured by chain, top view.
Source organism: Neisseria polysaccharea
Primary publication:
Oligosaccharide and sucrose complexes of amylosucrase. Structural implications for the polymerase activity.
Skov LK, Mirza O, Sprogøe D, Dar I, Remaud-Simeon M, Albenne C, Monsan P, Gajhede M
J Biol Chem 277 47741-7 (2002)
PMID: 12364331

Function and Biology Details

Reaction catalysed: 
Sucrose + ((1->4)-alpha-D-glucosyl)(n) = D-fructose + ((1->4)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195625 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Amylosucrase Chain: A
Molecule details ›
Chain: A
Length: 628 amino acids
Theoretical weight: 71.56 KDa
Source organism: Neisseria polysaccharea
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZEU2 (Residues: 13-636; Coverage: 98%)
Gene name: ams
Sequence domains: Alpha amylase, catalytic domain
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
1 bound ligand:
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P21212
Unit cell:
a: 97.409Å b: 115.81Å c: 61.136Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.227
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structure-function relationships of glucansucrase and fructansucrase enzymes from lactic acid bacteria.
van Hijum et al. (2006)
3 more

1 mention without citation

Insight into the structure, dynamics and the unfolding property of amylosucrases: implications of rational engineering on thermostability.
Liu et al. (2012)