› 1mw8

X-ray diffraction
1.9Å resolution

Crystal Structure of a Complex between H365R mutant of 67 kDA N-terminal fragment of E. coli DNA Topoisomerase I and 5'-ACTTCGGGATG-3'

Released:
DOI: 10.2210/pdb1mw8/pdb
PDB entry 1mw8 coloured by chain, front view.
PDB entry 1mw8 coloured by chain, side view.
PDB entry 1mw8 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of a complex between E. coli DNA topoisomerase I and single-stranded DNA.
Perry K, Mondragón A
Structure 11 1349-58 (2003)
PMID: 14604525

Function and Biology Details

Reaction catalysed: 
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114208 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA topoisomerase 1 Chain: X
Molecule details ›
Chain: X
Length: 592 amino acids
Theoretical weight: 66.93 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06612 (Residues: 1-592; Coverage: 68%)
Gene names: JW1266, b1274, supX, topA
Sequence domains:
Structure domains:
5'-D(*AP*CP*TP*TP*CP*GP*GP*GP*AP*TP*G)-3' Chain: Y
Molecule details ›
Chain: Y
Length: 11 nucleotides
Theoretical weight: 3.39 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand TMP 1 x TMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P212121
Unit cell:
a: 63.392Å b: 79.148Å c: 141.798Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.223 0.273
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

Drugging topoisomerases: lessons and challenges.
Pommier Y. (2013)
5 more

12 mentions without citation

Mechanism of Type IA Topoisomerases.
Dasgupta et al. (2020)
11 more