› 1mw9

X-ray diffraction
1.67Å resolution

Crystal Structure of H365R mutant of 67 kDA N-terminal fragment of E. coli DNA Topoisomerase I

Released:
DOI: 10.2210/pdb1mw9/pdb
PDB entry 1mw9 coloured by chain, front view.
PDB entry 1mw9 coloured by chain, side view.
PDB entry 1mw9 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of a complex between E. coli DNA topoisomerase I and single-stranded DNA.
Perry K, Mondragón A
Structure 11 1349-58 (2003)
PMID: 14604525

Function and Biology Details

Reaction catalysed: 
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139094 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 1 Chain: X
Molecule details ›
Chain: X
Length: 592 amino acids
Theoretical weight: 66.93 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06612 (Residues: 1-592; Coverage: 68%)
Gene names: JW1266, b1274, supX, topA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 9 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 62.99Å b: 78.705Å c: 139.328Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.216
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Drugging topoisomerases: lessons and challenges.
Pommier Y. (2013)
7 more

5 mentions without citation

Predictive Power of In Silico Approach to Evaluate Chemicals against M. tuberculosis: A Systematic Review.
Timo et al. (2019)
4 more