1mx1

X-ray diffraction
2.4Å resolution

Crystal Structure of Human Liver Carboxylesterase in complex with tacrine

Released:

Function and Biology Details

Reactions catalysed:
A carboxylic ester + H(2)O = an alcohol + a carboxylate
A steryl ester + H(2)O = a sterol + a fatty acid
4-methylumbelliferyl acetate + H(2)O = 4-methylumbelliferone + acetate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
PDBe Complex ID:
PDB-CPX-149883 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Liver carboxylesterase 1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 548 amino acids
Theoretical weight: 60.51 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P23141 (Residues: 19-567; Coverage: 100%)
Gene names: CES1, CES2, SES1
Sequence domains: Carboxylesterase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, NDG
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 90.021Å b: 117.03Å c: 176.01Å
α: 90° β: 95.69° γ: 90°
R-values:
R R work R free
0.184 0.162 0.207
Expression system: Spodoptera frugiperda