1mx5

X-ray diffraction
2.8Å resolution

Crystal Structure of Human Liver Carboxylesterase in complexed with homatropine, a cocaine analogue

Released:

Function and Biology Details

Reactions catalysed:
A carboxylic ester + H(2)O = an alcohol + a carboxylate
A steryl ester + H(2)O = a sterol + a fatty acid
4-methylumbelliferyl acetate + H(2)O = 4-methylumbelliferone + acetate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
PDBe Complex ID:
PDB-CPX-149882 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Liver carboxylesterase 1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 548 amino acids
Theoretical weight: 60.51 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P23141 (Residues: 19-567; Coverage: 100%)
Gene names: CES1, CES2, SES1
Sequence domains: Carboxylesterase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 55.4Å b: 178.8Å c: 199.6Å
α: 90° β: 90.2° γ: 90°
R-values:
R R work R free
0.19 0.158 0.221
Expression system: Spodoptera frugiperda