› 1my5

X-ray diffraction
1.8Å resolution

NF-kappaB p65 subunit dimerization domain homodimer

Released:
DOI: 10.2210/pdb1my5/pdb
PDB entry 1my5 coloured by chain, front view.
PDB entry 1my5 coloured by chain, side view.
PDB entry 1my5 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Solvent exposed non-contacting amino acids play a critical role in NF-kappaB/IkappaBalpha complex formation.
Huxford T, Mishler D, Phelps CB, Huang DB, Sengchanthalangsy LL, Reeves R, Hughes CA, Komives EA, Ghosh G
J Mol Biol 324 587-97 (2002)
PMID: 12460563

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170005 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription factor p65 Chains: A, B
Molecule details ›
Chains: A, B
Length: 114 amino acids
Theoretical weight: 13.2 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q04207 (Residues: 191-304; Coverage: 21%)
Gene names: Nfkb3, Rela
Sequence domains: Rel homology dimerisation domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2221
Unit cell:
a: 56.348Å b: 74.732Å c: 109.147Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.2 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Dynamic Protein Interaction Networks and New Structural Paradigms in Signaling.
Csizmok et al. (2016)
5 more

10 mentions without citation

Genome reading by the NF-κB transcription factors.
Mulero et al. (2019)
9 more