› 1mzg

X-ray diffraction
2Å resolution

X-Ray Structure of SufE from E.coli Northeast Structural Genomics (NESG) Consortium Target ER30

Released:
DOI: 10.2210/pdb1mzg/pdb
PDB entry 1mzg coloured by chain, front view.
PDB entry 1mzg coloured by chain, side view.
PDB entry 1mzg coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The SufE sulfur-acceptor protein contains a conserved core structure that mediates interdomain interactions in a variety of redox protein complexes.
Goldsmith-Fischman S, Kuzin A, Edstrom WC, Benach J, Shastry R, Xiao R, Acton TB, Honig B, Montelione GT, Hunt JF
J Mol Biol 344 549-65 (2004)
PMID: 15522304

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160063 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine desulfuration protein SufE Chains: A, B
Molecule details ›
Chains: A, B
Length: 146 amino acids
Theoretical weight: 17.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P76194 (Residues: 1-138; Coverage: 100%)
Gene names: JW1669, b1679, sufE, ynhA
Sequence domains: Fe-S metabolism associated domain
Structure domains: Sufe protein. Chain: A

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 42.19Å b: 54.401Å c: 120.685Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Fe-S cluster assembly pathways in bacteria.
Ayala-Castro et al. (2008)
9 more

12 mentions without citation

Interplay between oxygen and Fe-S cluster biogenesis: insights from the Suf pathway.
Boyd et al. (2014)
11 more