› 1n05

X-ray diffraction
2.1Å resolution

Crystal Structure of Schizosaccharomyces pombe Riboflavin Kinase Reveals a Novel ATP and Riboflavin Binding Fold

Released:
DOI: 10.2210/pdb1n05/pdb
PDB entry 1n05 coloured by chain, front view.
PDB entry 1n05 coloured by chain, side view.
PDB entry 1n05 coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
Crystal structure of Schizosaccharomyces pombe riboflavin kinase reveals a novel ATP and riboflavin-binding fold.
Bauer S, Kemter K, Bacher A, Huber R, Fischer M, Steinbacher S
J Mol Biol 326 1463-73 (2003)
PMID: 12595258

Function and Biology Details

Reaction catalysed: 
ATP + riboflavin = ADP + FMN
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130800 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Riboflavin kinase Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 18.94 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: O74866 (Residues: 1-163; Coverage: 100%)
Gene names: SPCC18.16c, fmn1
Sequence domains: Riboflavin kinase
Structure domains: Riboflavin kinase-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P6122
Unit cell:
a: 69.497Å b: 69.497Å c: 139.851Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.221 0.267
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Genetic control of biosynthesis and transport of riboflavin and flavin nucleotides and construction of robust biotechnological producers.
Abbas et al. (2011)
4 more

2 mentions without citation

Evolutionary divergence of chloroplast FAD synthetase proteins.
Yruela et al. (2010)
1 more