› 1n0s

X-ray diffraction
2Å resolution

ENGINEERED LIPOCALIN FLUA IN COMPLEX WITH FLUORESCEIN

Released:
DOI: 10.2210/pdb1n0s/pdb
PDB entry 1n0s coloured by chain, front view.
PDB entry 1n0s coloured by chain, side view.
PDB entry 1n0s coloured by chain, top view.
Source organism: Pieris brassicae
Primary publication:
Crystallographic analysis of an "anticalin" with tailored specificity for fluorescein reveals high structural plasticity of the lipocalin loop region.
Korndörfer IP, Beste G, Skerra A
Proteins 53 121-9 (2003)
PMID: 12945055

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140658 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bilin-binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 21.06 KDa
Source organism: Pieris brassicae
Expression system: Escherichia coli
UniProt:
  • Canonical: P09464 (Residues: 16-189; Coverage: 100%)
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand FLU 2 x FLU
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 42.797Å b: 85.039Å c: 66.059Å
α: 90° β: 106.18° γ: 90°
R-values:
R R work R free
0.196 0.193 0.243
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Engineering novel binding proteins from nonimmunoglobulin domains.
Binz et al. (2005)
10 more

9 mentions without citation

Structural insights for engineering binding proteins based on non-antibody scaffolds.
Gilbreth et al. (2012)
8 more