› 1n12

X-ray diffraction
1.87Å resolution

Crystal structure of the PapE (N-terminal-deleted) pilus subunit bound to a peptide corresponding to the N-terminal extension of the PapK pilus subunit (residues 1-11) from uropathogenic E. coli

Released:
DOI: 10.2210/pdb1n12/pdb
PDB entry 1n12 coloured by chain, front view.
PDB entry 1n12 coloured by chain, side view.
PDB entry 1n12 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Chaperone priming of pilus subunits facilitates a topological transition that drives fiber formation.
Sauer FG, Pinkner JS, Waksman G, Hultgren SJ
Cell 111 543-51 (2002)
PMID: 12437927

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fimbrial protein PapE Chains: A, C
Molecule details ›
Chains: A, C
Length: 138 amino acids
Theoretical weight: 14.83 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P08407 (Residues: 36-173; Coverage: 93%)
Gene name: papE
Sequence domains: Fimbrial protein
Structure domains: Fimbrial-type adhesion domain
Protein PrsK Chains: B, D
Molecule details ›
Chains: B, D
Length: 11 amino acids
Theoretical weight: 1.21 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P42191 (Residues: 22-32; Coverage: 7%)
Gene name: prsK

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 25.254Å b: 91.922Å c: 51.244Å
α: 90° β: 99.26° γ: 90°
R-values:
R R work R free
0.211 0.208 0.249
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

41 review citations

The unfolding story of three-dimensional domain swapping.
Rousseau et al. (2003)
40 more

10 mentions without citation

A tale of two pili: assembly and function of pili in bacteria.
Kline et al. (2010)
9 more