› 1n1p

X-ray diffraction
0.95Å resolution

ATOMIC RESOLUTION STRUCTURE OF CHOLESTEROL OXIDASE @ pH 7.4 (STREPTOMYCES SP. SA-COO)

Released:
DOI: 10.2210/pdb1n1p/pdb
PDB entry 1n1p coloured by chain, front view.
PDB entry 1n1p coloured by chain, side view.
PDB entry 1n1p coloured by chain, top view.
Source organism: Streptomyces sp.
Primary publication:
Atomic resolution density maps reveal secondary structure dependent differences in electronic distribution.
Lario PI, Vrielink A
J Am Chem Soc 125 12787-94 (2003)
PMID: 14558826

Function and Biology Details

Reactions catalysed: 
Cholesterol + O(2) = cholest-5-en-3-one + H(2)O(2)
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146297 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cholesterol oxidase Chain: A
Molecule details ›
Chain: A
Length: 504 amino acids
Theoretical weight: 54.97 KDa
Source organism: Streptomyces sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: P12676 (Residues: 43-546; Coverage: 100%)
Gene name: choA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
2 bound ligands:
Ligand MN 3 x MN
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21
Unit cell:
a: 51.273Å b: 72.964Å c: 63.036Å
α: 90° β: 105.18° γ: 90°
R-values:
R R work R free
0.097 0.097 0.119
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Energetics of protein folding.
Baldwin RL. (2007)
12 other articles

7 mentions without citation

Automated ligand fitting by core-fragment fitting and extension into density.
Terwilliger et al. (2006)
6 more