› 1n2c

X-ray diffraction
3Å resolution

NITROGENASE COMPLEX FROM AZOTOBACTER VINELANDII STABILIZED BY ADP-TETRAFLUOROALUMINATE

Released:
DOI: 10.2210/pdb1n2c/pdb
PDB entry 1n2c coloured by chain, front view.
PDB entry 1n2c coloured by chain, side view.
PDB entry 1n2c coloured by chain, top view.
Source organism: Azotobacter vinelandii
Primary publication:
Structure of ADP x AIF4(-)-stabilized nitrogenase complex and its implications for signal transduction.
Schindelin H, Kisker C, Schlessman JL, Howard JB, Rees DC
Nature 387 370-6 (1997)
PMID: 9163420

Function and Biology Details

Reaction catalysed: 
8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132541 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Nitrogenase molybdenum-iron protein alpha chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 491 amino acids
Theoretical weight: 55.23 KDa
Source organism: Azotobacter vinelandii
UniProt:
  • Canonical: P07328 (Residues: 2-492; Coverage: 100%)
Gene name: nifD
Sequence domains: Nitrogenase component 1 type Oxidoreductase
Structure domains: Nitrogenase molybdenum iron protein domain
Nitrogenase molybdenum-iron protein beta chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 522 amino acids
Theoretical weight: 59.4 KDa
Source organism: Azotobacter vinelandii
UniProt:
  • Canonical: P07329 (Residues: 2-523; Coverage: 100%)
Gene name: nifK
Sequence domains:
Structure domains:
Nitrogenase iron protein 1 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 289 amino acids
Theoretical weight: 31.42 KDa
Source organism: Azotobacter vinelandii
UniProt:
  • Canonical: P00459 (Residues: 2-290; Coverage: 100%)
Gene name: nifH1
Sequence domains: 4Fe-4S iron sulfur cluster binding proteins, NifH/frxC family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

8 bound ligands:
Ligand HCA 2 x HCA
Ligand CFM 2 x CFM
Ligand CA 2 x CA
Ligand CLF 2 x CLF
Ligand MG 4 x MG
Ligand ALF 4 x ALF
Ligand ADP 4 x ADP
Ligand SF4 2 x SF4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: C2221
Unit cell:
a: 79Å b: 299.7Å c: 334.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.238

Quick links

Citations

53 review citations

ABC transporters: the power to change.
Rees et al. (2009)
52 more

38 mentions without citation

Metalloproteins containing cytochrome, iron-sulfur, or copper redox centers.
Liu et al. (2014)
37 more