› 1n3b

X-ray diffraction
1.8Å resolution

Crystal Structure of Dephosphocoenzyme A kinase from Escherichia coli

Released:
DOI: 10.2210/pdb1n3b/pdb
PDB entry 1n3b coloured by chain, front view.
PDB entry 1n3b coloured by chain, side view.
PDB entry 1n3b coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of a trimeric form of dephosphocoenzyme A kinase from Escherichia coli.
O'Toole N, Barbosa JA, Li Y, Hung LW, Matte A, Cygler M
Protein Sci 12 327-36 (2003)
PMID: 12538896

Function and Biology Details

Reaction catalysed: 
ATP + 3'-dephospho-CoA = ADP + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141364 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dephospho-CoA kinase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 216 amino acids
Theoretical weight: 24.04 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6I9 (Residues: 1-206; Coverage: 100%)
Gene names: JW0100, b0103, coaE, yacE
Sequence domains: Dephospho-CoA kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand SO4 10 x SO4
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21
Unit cell:
a: 55.5Å b: 82.41Å c: 76Å
α: 90° β: 94.77° γ: 90°
R-values:
R R work R free
0.217 0.217 0.246
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Coenzyme A biosynthesis: an antimicrobial drug target.
Spry et al. (2008)
2 more

5 mentions without citation

Contribution of structural genomics to understanding the biology of Escherichia coli.
Matte et al. (2003)
4 more