› 1n47

X-ray diffraction
2.7Å resolution

Isolectin B4 from Vicia villosa in complex with the Tn antigen

Released:
DOI: 10.2210/pdb1n47/pdb
PDB entry 1n47 coloured by chain, front view.
PDB entry 1n47 coloured by chain, side view.
PDB entry 1n47 coloured by chain, top view.
Source organism: Vicia villosa
Primary publication:
The crystal structure of a plant lectin in complex with the Tn antigen.
Babino A, Tello D, Rojas A, Bay S, Osinaga E, Alzari PM
FEBS Lett 536 106-10 (2003)
PMID: 12586347

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157501 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lectin B4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 233 amino acids
Theoretical weight: 24.71 KDa
Source organism: Vicia villosa
UniProt:
  • Canonical: P56625 (Residues: 1-36, 37-47, 48-149, 150-160, 161-185; Coverage: 100%)
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
4 bound ligands:
Ligand CA 4 x CA
Ligand MN 4 x MN
Ligand SER 4 x SER
Ligand A2G 4 x A2G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32
Spacegroup: P43
Unit cell:
a: 85.51Å b: 85.51Å c: 153.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.238

Quick links

Citations

1 review citation

Legume Lectins: Proteins with Diverse Applications.
Lagarda-Diaz et al. (2017)
26 other articles

4 mentions without citation

Plant Lectins Targeting O-Glycans at the Cell Surface as Tools for Cancer Diagnosis, Prognosis and Therapy.
Poiroux et al. (2017)
3 more