› 1n5s

X-ray diffraction
1.7Å resolution

Crystal structure of a Monooxygenase from the gene ActVA-Orf6 of Streptomyces coelicolor in complex with the ligand Acetyl Dithranol

Released:
DOI: 10.2210/pdb1n5s/pdb
PDB entry 1n5s coloured by chain, front view.
PDB entry 1n5s coloured by chain, side view.
PDB entry 1n5s coloured by chain, top view.
Source organism: Streptomyces coelicolor A3(2)
Primary publication:
The structure of ActVA-Orf6, a novel type of monooxygenase involved in actinorhodin biosynthesis.
Sciara G, Kendrew SG, Miele AE, Marsh NG, Federici L, Malatesta F, Schimperna G, Savino C, Vallone B
EMBO J 22 205-15 (2003)
PMID: 12514126

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176084 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ABM domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 112 amino acids
Theoretical weight: 11.98 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53908 (Residues: 2-113; Coverage: 99%)
Gene name: actVA 6
Sequence domains: Antibiotic biosynthesis monooxygenase
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand 1PG 1 x 1PG
Ligand ADL 1 x ADL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P212121
Unit cell:
a: 47.082Å b: 59.677Å c: 71.648Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.246
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Flavoprotein monooxygenases, a diverse class of oxidative biocatalysts.
van Berkel et al. (2006)
17 more

1 mention without citation

The structure of ActVA-Orf6, a novel type of monooxygenase involved in actinorhodin biosynthesis.
Sciara et al. (2003)