› 1n87

Solution NMR

Solution structure of the U-box of Prp19

Released:
DOI: 10.2210/pdb1n87/pdb
PDB entry 1n87 coloured by chain, ensemble of 20 models, front view.
PDB entry 1n87 coloured by chain, ensemble of 20 models, side view.
PDB entry 1n87 coloured by chain, ensemble of 20 models, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural insights into the U-box, a domain associated with multi-ubiquitination.
Ohi MD, Vander Kooi CW, Rosenberg JA, Chazin WJ, Gould KL
Nat Struct Biol 10 250-5 (2003)
PMID: 12627222

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152347 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pre-mRNA-processing factor 19 Chain: A
Molecule details ›
Chain: A
Length: 56 amino acids
Theoretical weight: 6.25 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P32523 (Residues: 1-56; Coverage: 11%)
Gene names: PRP19, PSO4, YLL036C
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: Torsion angle dynamics, Molecular dynamics
Expression system: Escherichia coli BL21

Quick links

Citations

71 review citations

Ubiquitin: structures, functions, mechanisms.
Pickart et al. (2004)
70 more

3 mentions without citation

Structural insights into the U-box, a domain associated with multi-ubiquitination.
Ohi et al. (2003)
2 more