› 1n97

X-ray diffraction
1.8Å resolution

Crystal Structure of CYP175A1 from Thermus thermophillus strain HB27

Released:
DOI: 10.2210/pdb1n97/pdb
PDB entry 1n97 coloured by chain, front view.
PDB entry 1n97 coloured by chain, side view.
PDB entry 1n97 coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Preliminary characterization and crystal structure of a thermostable cytochrome P450 from Thermus thermophilus.
Yano JK, Blasco F, Li H, Schmid RD, Henne A, Poulos TL
J Biol Chem 278 608-16 (2003)
PMID: 12401810

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181006 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 Chains: A, B
Molecule details ›
Chains: A, B
Length: 389 amino acids
Theoretical weight: 44.32 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q746J6 (Residues: 1-389; Coverage: 100%)
Gene name: TT_P0059
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

3 bound ligands:
Ligand SRT 2 x SRT
Ligand HEM 2 x HEM
Ligand EDO 18 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 64.538Å b: 90.699Å c: 74.516Å
α: 90° β: 99.68° γ: 90°
R-values:
R R work R free
0.207 0.182 0.222
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Hydrocarbon hydroxylation by cytochrome P450 enzymes.
Ortiz de Montellano PR. (2010)
7 more

3 mentions without citation

Conformational plasticity and structure/function relationships in cytochromes P450.
Pochapsky et al. (2010)
2 more