› 1n9n

X-ray diffraction
2.3Å resolution

Crystal structure of the Phot-LOV1 domain from Chlamydomonas reinhardtii in illuminated state. Data set of a single crystal.

Released:
DOI: 10.2210/pdb1n9n/pdb
PDB entry 1n9n coloured by chain, front view.
PDB entry 1n9n coloured by chain, side view.
PDB entry 1n9n coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Crystal structures and molecular mechanism of a light-induced signaling switch: The Phot-LOV1 domain from Chlamydomonas reinhardtii.
Fedorov R, Schlichting I, Hartmann E, Domratcheva T, Fuhrmann M, Hegemann P
Biophys J 84 2474-82 (2003)
PMID: 12668455

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185298 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phototropin Chain: A
Molecule details ›
Chain: A
Length: 109 amino acids
Theoretical weight: 11.99 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8LPD9 (Residues: 17-125; Coverage: 15%)
Gene names: CHLREDRAFT_183965, CHLRE_03g199000v5, PHOT
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P6522
Unit cell:
a: 120.9Å b: 120.9Å c: 46Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.212 0.265
Expression system: Escherichia coli BL21

Quick links

Citations

26 review citations

Phytochrome structure and signaling mechanisms.
Rockwell et al. (2006)
25 more

5 mentions without citation

Crystal structures and molecular mechanism of a light-induced signaling switch: The Phot-LOV1 domain from Chlamydomonas reinhardtii.
Fedorov et al. (2003)
4 more