› 1n9r

X-ray diffraction
2.8Å resolution

Crystal structure of a heptameric ring complex of yeast SmF in spacegroup P4122

Released:
DOI: 10.2210/pdb1n9r/pdb
PDB entry 1n9r coloured by chain, front view.
PDB entry 1n9r coloured by chain, side view.
PDB entry 1n9r coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Homomeric ring assemblies of eukaryotic Sm proteins have affinity for both RNA and DNA. Crystal structure of an oligomeric complex of yeast SmF.
Collins BM, Cubeddu L, Naidoo N, Harrop SJ, Kornfeld GD, Dawes IW, Curmi PM, Mabbutt BC
J Biol Chem 278 17291-8 (2003)
PMID: 12618433

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo heptamer (preferred)
homo tetradecamer
Assembly name:
PDBe Complex ID:
PDB-CPX-157144 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Small nuclear ribonucleoprotein F Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 93 amino acids
Theoretical weight: 10.63 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P54999 (Residues: 1-86; Coverage: 100%)
Gene names: P9705.4, SMX3, YPR182W
Sequence domains: LSM domain
Structure domains: SH3 type barrels.

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P4122
Unit cell:
a: 79.891Å b: 79.891Å c: 251.188Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.254 0.254 0.268
Expression system: Escherichia coli

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Citations

2 review citations

Survey of the year 2003 commercial optical biosensor literature.
Rich et al. (2005)
1 more

4 mentions without citation

Lessons from making the Structural Classification of Proteins (SCOP) and their implications for protein structure modelling.
Andreeva A. (2016)
3 more