› 1na6

X-ray diffraction
2.1Å resolution

Crystal structure of restriction endonuclease EcoRII mutant R88A

Released:
DOI: 10.2210/pdb1na6/pdb
PDB entry 1na6 coloured by chain, front view.
PDB entry 1na6 coloured by chain, side view.
PDB entry 1na6 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of type IIE restriction endonuclease EcoRII reveals an autoinhibition mechanism by a novel effector-binding fold.
Zhou XE, Wang Y, Reuter M, Mücke M, Krüger DH, Meehan EJ, Chen L
J Mol Biol 335 307-19 (2004)
PMID: 14659759

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146987 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II restriction enzyme EcoRII Chains: A, B
Molecule details ›
Chains: A, B
Length: 404 amino acids
Theoretical weight: 45.46 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P14633 (Residues: 3-404; Coverage: 100%)
Gene name: ecoRIIR
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 58.746Å b: 92.36Å c: 88.308Å
α: 90° β: 108.08° γ: 90°
R-values:
R R work R free
0.246 0.23 0.284
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structures and evolutionary origins of plant-specific transcription factor DNA-binding domains.
Yamasaki et al. (2008)
1 more

7 mentions without citation

Identification of novel restriction endonuclease-like fold families among hypothetical proteins.
Kinch et al. (2005)
6 more