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Solution NMR

SOLUTION STRUCTURE OF AN RNA 2'-O-METHYLATED RNA DUPLEX CONTAINING AN RNA/DNA HYBRID SEGMENT AT THE CENTER, NMR, MINIMIZED AVERAGE STRUCTURE

Released:
DOI: 10.2210/pdb1nao/pdb
PDB entry 1nao coloured by chain, front view.
PDB entry 1nao coloured by chain, side view.
PDB entry 1nao coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Solution structure of an RNA.2'-O-methylated RNA hybrid duplex containing an RNA.DNA hybrid segment at the center.
Nishizaki T, Iwai S, Ohtsuka E, Nakamura H
Biochemistry 36 2577-85 (1997)
PMID: 9054564

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-113085 (preferred)
Entry contents:
1 distinct RNA molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (2 distinct):
RNA (5'-R(*GP*GP*AP*GP*AP*UP*GP*AP*C)-3') Chain: A
Molecule details ›
Chain: A
Length: 9 nucleotides
Theoretical weight: 2.93 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA/RNA (5'-R(*OMGP*OMUP*OMC)-D(P*AP*TP*CP*T)-R(P*OMCP*OMC)-3') Chain: B
Molecule details ›
Chain: B
Length: 9 nucleotides
Theoretical weight: 2.8 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
3 modified residues:
Ligand OMG 1 x OMG
Ligand OMU 1 x OMU
Ligand OMC 3 x OMC

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Not provided

Quick links

Citations

3 review citations

Designing chemically modified oligonucleotides for targeted gene silencing.
Deleavey et al. (2012)
2 more

1 mention without citation

The crystal structure of the Sgt1-Skp1 complex: the link between Hsp90 and both SCF E3 ubiquitin ligases and kinetochores.
Willhoft et al. (2017)