1nb4

X-ray diffraction
2Å resolution

HC-J4 RNA polymerase apo-form

Released:
Model geometry
Fit model/data
Data not deposited

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-131655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 570 amino acids
Theoretical weight: 63.46 KDa
Source organism: Hepacivirus hominis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O92972 (Residues: 2420-2989; Coverage: 19%)
Sequence domains: Viral RNA dependent RNA polymerase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P212121
Unit cell:
a: 106.69Å b: 108.71Å c: 135.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.204 0.246
Expression system: Escherichia coli BL21