› 1nep

X-ray diffraction
1.7Å resolution

Crystal Structure Analysis of the Bovine NPC2 (Niemann-Pick C2) Protein

Released:
DOI: 10.2210/pdb1nep/pdb
PDB entry 1nep coloured by chain, front view.
PDB entry 1nep coloured by chain, side view.
PDB entry 1nep coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structure of a cholesterol-binding protein deficient in Niemann-Pick type C2 disease.
Friedland N, Liou HL, Lobel P, Stock AM
Proc Natl Acad Sci U S A 100 2512-7 (2003)
PMID: 12591954

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160320 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NPC intracellular cholesterol transporter 2 Chain: A
Molecule details ›
Chain: A
Length: 130 amino acids
Theoretical weight: 14.63 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P79345 (Residues: 20-149; Coverage: 100%)
Gene name: NPC2
Sequence domains: ML domain
Structure domains: Immunoglobulin-like

Ligands and Environments

2 bound ligands:
Ligand NAG 1 x NAG
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: I23
Unit cell:
a: 100.157Å b: 100.157Å c: 100.157Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 0.212

Quick links

Citations

47 review citations

Role of cholesterol and lipid organization in disease.
Maxfield et al. (2005)
46 more

15 mentions without citation

Snapshots of ABCG1 and ABCG5/G8: A Sterol's Journey to Cross the Cellular Membranes.
Rezaei et al. (2022)
14 more