› 1nez

X-ray diffraction
2.1Å resolution

The Crystal Structure of a TL/CD8aa Complex at 2.1A resolution:Implications for Memory T cell Generation, Co-receptor Preference and Affinity

Released:
DOI: 10.2210/pdb1nez/pdb
PDB entry 1nez coloured by chain, front view.
PDB entry 1nez coloured by chain, side view.
PDB entry 1nez coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The crystal structure of a TL/CD8alphaalpha complex at 2.1 A resolution: implications for modulation of T cell activation and memory.
Liu Y, Xiong Y, Naidenko OV, Liu JH, Zhang R, Joachimiak A, Kronenberg M, Cheroutre H, Reinherz EL, Wang JH
Immunity 18 205-15 (2003)
PMID: 12594948

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134574 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, TLA(B) alpha chain Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.66 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P14432 (Residues: 27-300; Coverage: 77%)
Gene name: H2-T3
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
T-cell surface glycoprotein CD8 alpha chain Chains: G, H
Molecule details ›
Chains: G, H
Length: 128 amino acids
Theoretical weight: 14.4 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P01731 (Residues: 28-149; Coverage: 56%)
Gene names: Cd8a, Lyt-2, Lyt2
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3121
Unit cell:
a: 77.018Å b: 77.018Å c: 176.045Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.217 0.279
Expression system: Not provided

Quick links

Citations

27 review citations

How TCRs bind MHCs, peptides, and coreceptors.
Rudolph et al. (2006)
26 more

5 mentions without citation

Structural basis of the CD8 alpha beta/MHC class I interaction: focused recognition orients CD8 beta to a T cell proximal position.
Wang et al. (2009)
4 more