› 1nf2

X-ray diffraction
2.2Å resolution

X-ray crystal structure of TM0651 from Thermotoga maritima

Released:
DOI: 10.2210/pdb1nf2/pdb
PDB entry 1nf2 coloured by chain, front view.
PDB entry 1nf2 coloured by chain, side view.
PDB entry 1nf2 coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Crystal structure of a phosphatase with a unique substrate binding domain from Thermotoga maritima.
Shin DH, Roberts A, Jancarik J, Yokota H, Kim R, Wemmer DE, Kim SH
Protein Sci 12 1464-72 (2003)
PMID: 12824492

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-194588 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
phosphatase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 268 amino acids
Theoretical weight: 31.23 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WZB9 (Residues: 1-268; Coverage: 100%)
Gene name: TM_0651
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 6 x SO4
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: C2
Unit cell:
a: 158.41Å b: 93.88Å c: 98.42Å
α: 90° β: 127.91° γ: 90°
R-values:
R R work R free
0.222 0.217 0.263
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Microbial biochemistry, physiology, and biotechnology of hyperthermophilic Thermotoga species.
Conners et al. (2006)
2 more

9 mentions without citation

Decision-making in structure solution using Bayesian estimates of map quality: the PHENIX AutoSol wizard.
Terwilliger et al. (2009)
8 more