› 1ng5

X-ray diffraction
2Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101932 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NPQTN specific sortase B Chains: A, B
Molecule details ›
Chains: A, B
Length: 215 amino acids
Theoretical weight: 25.64 KDa
Source organism: Staphylococcus aureus subsp. aureus N315
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3JPP1 (Residues: 30-244; Coverage: 88%)
Gene name: srtB
Sequence domains: Sortase domain
Structure domains: Sortase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 71.158Å b: 104.383Å c: 58.158Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.237 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Sortases and the art of anchoring proteins to the envelopes of gram-positive bacteria.
Marraffini et al. (2006)
13 more

18 mentions without citation

Sortase enzymes in Gram-positive bacteria.
Spirig et al. (2011)
17 more