› 1ngw

X-ray diffraction
2.6Å resolution

Chimeric Affinity Matured Fab 7g12 complexed with mesoporphyrin

Released:
DOI: 10.2210/pdb1ngw/pdb
PDB entry 1ngw coloured by chain, front view.
PDB entry 1ngw coloured by chain, side view.
PDB entry 1ngw coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural evidence for substrate strain in antibody catalysis.
Yin J, Andryski SE, Beuscher AE, Stevens RC, Schultz PG
Proc Natl Acad Sci U S A 100 856-61 (2003)
PMID: 12552112

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209280 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mature Metal Chelatase Catalytic Antibody, Light chain Chains: A, L
Molecule details ›
Chains: A, L
Length: 213 amino acids
Theoretical weight: 23.43 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Ig heavy chain V region 1-72 Chains: B, H
Molecule details ›
Chains: B, H
Length: 216 amino acids
Theoretical weight: 23.1 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P06328 (Residues: 20-117; Coverage: 100%)
Gene name: Ighv1-72
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand MMP 2 x MMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21212
Unit cell:
a: 100.721Å b: 134.215Å c: 72.951Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.275 0.275 0.318
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Mechanical processes in biochemistry.
Bustamante et al. (2004)
2 more

5 mentions without citation

Chelatases: distort to select?
Al-Karadaghi et al. (2006)
4 more