› 1nh7

X-ray diffraction
2.7Å resolution

ATP PHOSPHORIBOSYLTRANSFERASE (ATP-PRTASE) FROM MYCOBACTERIUM TUBERCULOSIS

Released:
DOI: 10.2210/pdb1nh7/pdb
PDB entry 1nh7 coloured by chain, front view.
PDB entry 1nh7 coloured by chain, side view.
PDB entry 1nh7 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Crystal structure of ATP phosphoribosyltransferase from Mycobacterium tuberculosis.
Cho Y, Sharma V, Sacchettini JC
J Biol Chem 278 8333-9 (2003)
PMID: 12511575

Function and Biology Details

Reaction catalysed: 
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = ATP + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-161811 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP phosphoribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 304 amino acids
Theoretical weight: 32.68 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WMN1 (Residues: 1-284; Coverage: 100%)
Gene names: MTCY261.17c, Rv2121c, hisG
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C, null
Spacegroup: R32
Unit cell:
a: 132.54Å b: 132.54Å c: 110.5Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.192 0.261
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

The structural basis of allosteric regulation in proteins.
Laskowski et al. (2009)
7 more

7 mentions without citation

Insight into de-regulation of amino acid feedback inhibition: a focus on structure analysis method.
Naz et al. (2023)
6 more