› 1ni5

X-ray diffraction
2.65Å resolution

Structure of the MesJ PP-ATPase from Escherichia Coli

Released:
DOI: 10.2210/pdb1ni5/pdb
PDB entry 1ni5 coloured by chain, front view.
PDB entry 1ni5 coloured by chain, side view.
PDB entry 1ni5 coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Gu M, Burling T, Lima CD, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156467 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA(Ile)-lysidine synthase Chain: A
Molecule details ›
Chain: A
Length: 433 amino acids
Theoretical weight: 48.58 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P52097 (Residues: 1-432; Coverage: 100%)
Gene names: JW0183, b0188, mesJ, tilS, yaeN
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P3121
Unit cell:
a: 67.508Å b: 67.508Å c: 204.678Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.223 0.293
Expression system: Escherichia coli

Quick links