› 1njo

X-ray diffraction
3.7Å resolution

The crystal structure of the 50S Large ribosomal subunit from Deinococcus radiodurans complexed with a short substrate analog ACCPuromycin (ACCP)

Released:
DOI: 10.2210/pdb1njo/pdb
PDB entry 1njo coloured by chain, front view.
PDB entry 1njo coloured by chain, side view.
PDB entry 1njo coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of the ribosomal machinery for peptide bond formation, translocation, and nascent chain progression.
Bashan A, Agmon I, Zarivach R, Schluenzen F, Harms J, Berisio R, Bartels H, Franceschi F, Auerbach T, Hansen HA, Kossoy E, Kessler M, Yonath A
Mol Cell 11 91-102 (2003)
PMID: 12535524

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196141 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
23S ribosomal RNA Chain: 0
Molecule details ›
Chain: 0
Length: 2880 nucleotides
Theoretical weight: 933.41 KDa
Source organism: Deinococcus radiodurans
Sequence domains: Bacterial large subunit ribosomal RNA
RNA ACC(Puromycin) Chain: 5
Molecule details ›
Chain: 5
Length: 4 nucleotides
Theoretical weight: 1.43 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PPU 1 x PPU

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: I222
Unit cell:
a: 169.9Å b: 410.4Å c: 697.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.283 0.283 0.297
Expression system: Not provided

Quick links

Citations

46 review citations

What recent ribosome structures have revealed about the mechanism of translation.
Schmeing et al. (2009)
45 more

4 mentions without citation

POPSCOMP: an automated interaction analysis of biomolecular complexes.
Kleinjung et al. (2005)
3 more