› 1nl3

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF THE SECA PROTEIN TRANSLOCATION ATPASE FROM MYCOBACTERIUM TUBERCULOSIS in APO FORM

Released:
DOI: 10.2210/pdb1nl3/pdb
PDB entry 1nl3 coloured by chain, front view.
PDB entry 1nl3 coloured by chain, side view.
PDB entry 1nl3 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structure of Mycobacterium tuberculosis SecA, a preprotein translocating ATPase.
Sharma V, Arockiasamy A, Ronning DR, Savva CG, Holzenburg A, Braunstein M, Jacobs WR, Sacchettini JC
Proc Natl Acad Sci U S A 100 2243-8 (2003)
PMID: 12606717

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + cellular protein(Side 1) = ADP + phosphate + cellular protein(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-161506 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein translocase subunit SecA 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 922 amino acids
Theoretical weight: 103.46 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WGP5 (Residues: 2-892; Coverage: 94%)
Gene names: MTCY20B11.15c, Rv3240c, secA1
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6222
Unit cell:
a: 206.196Å b: 206.196Å c: 295.412Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.193 0.259
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

33 review citations

The DEAD-box protein family of RNA helicases.
Cordin et al. (2006)
32 more

16 mentions without citation

SecA, a remarkable nanomachine.
Kusters et al. (2011)
15 more